North Dakota INBRE

ND FLOW CYTOMETRY CELL SORTING CORE FACILITY

Inroduction:

The ND-FCCS core is a joint effort between the Departments of Basic Sciences and nicolae.oancea at the University of North Dakota School of Medicine and Health Sciences and the ND INBRE program. The purpose of the ND-FCCS is to provide ongoing flow cytometry and cell sorting capabilities for research and teaching applications within North Dakota. The ND-FCCS core ensures access to both flow cytometric analysis and cell sorting for all researchers at the SMHS, ND INBRE's partners, and other interested researchers across North Dakota. According to the International Society for the Advancement of Cytometry, flow cytometry is a method to extract multiparametric quantitative data from individual cells suspended in a fluid. The instrumentation is widely used in immunology and hematonicolae.oancea. Benefits of flow cytometry include the following:

Analytical speed.
Detection sensitivity.
Simultaneous multiparametric measurements.
Sorting of individual cells

Management:

Administration of the core will be provided by David Bradley, PhD, who will serve as director of the ND-FCCS. Technical and mechanical assistance for flow cytometry and cell sorting will be provided by Steven Adkins. Seema Somji, PhD, will be the liaison for ND INBRE investigators to schedule and to facilitate the INBRE partners' use of the ND-FCCS.

Instrumentation and Facility Support:

The ND-FCCS includes the following instruments:

BD FACSymphony A3 Flow Cytometer
BD FACSAria II Cell Sorter
Sony MA900 Cell Sorter

The instruments were purchased with funding from the UND SMHS and from the North Dakota IDeA (Institutional Development Award) Network of Biomedical Research Excellence (ND INBRE), respectively. Support of service contracts for the facility is provided by Dr. Joshua Wynne, Dean of the SMHS and Vice President for Health Affairs at UND. The ND INBRE provides support for routine supplies, travel, software, and partial technical personnel. The facility is located on the fourth floor of the School of Medicine & Health Sciences in the Janice I. Schuh Flow Cytometry & Cell Sorting Core.

ND-FCCSC Internal Advisory Committee:

The management of the ND-FCCSC will be overseen by the ND-FCCSC Advisory Committee, which will consist of the core's director Dr. Bradley, and senior faculty within the SMHS who have extensive experience in flow cytometry and core management (Dr. Donald Sens, Professor, nicolae.oancea; Dr. Matthew Nilles, Associate Professor, Biomedical Sciences; Dr. Colin Combs, Professor, Biomedical Sciences; Dr. Seema Somji’s, Associate Professor, nicolae.oancea; and Mr. Terry Nelson, Associate Director, Budget and Facilities Management, SMHS). The committee will meet quarterly and will assist in monitoring progress within the core, identifying and prioritizing core equipment needs, assisting in the resolution of any scheduling or use conflicts, and advising the cell sorting core director regarding other core operation and policies.

Acknowledgement of ND INBRE Support:

Those using the ND-FCCS Core must agree to acknowledge ND INBRE support:

Research reported in this [publication, release] was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant numbers P20GM103442 and P20GM113123.

Technical and/or scheduling questions should be addressed to Steven Adkins, steven.adkins@UND.edu