| Service |
Fee ($) |
Note |
| Sample preparation |
| SDS-PAGE gel electrophoresis |
$40/gel |
For separation of simple samples |
| 2D-Gel electrophoresis |
$150/gel |
For separation of complex samples |
| Gel image scanning |
$15/gel |
Accept gels stained with UV-visible or fluorescent dyes, e.g. Commassie blue and cypru by |
| Gel image analysis by PDQuest |
$50/hour |
For comparison of gels |
| In-gel digestion |
$20/sample for the first 6 samples, $10/sample afterward |
For digestion of proteins sepa-rated by SDS-PAGE or 2D-gel |
| In-solution digestion |
$15/sample |
For digestion of proteins in solution |
| Tube-gel digestion* |
$40/sample for the first 6 sample, $20/sample afterward |
For difficult samples, e.g. insoluble membrane proteins and samples with a high amount of detergents or salts. |
| Ziptip with C18, C8 or C4 stationary phase |
$15/sample |
For Sample Desalting and Enrichment |
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| Analysis with 4800 MALDI-TOF/TOF mass spectrometer |
| Peptide/protein molecular weight measurement |
$30/peptide sample
(< 5 kDa)
$60/protein sample
(> 5 kDa) |
For accurate mass measurement: 100 ppm for peptides; 500 ppm for proteins |
| MALDI-MS for Peptide fingerprinting |
$30/sample |
For protein identification and characterization |
| MALDI-MS/MS |
$60/sample |
For protein identification and characterization |
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| Analysis with QSTAR XL mass spectrometer |
| Infusion ESI-MS |
Please call |
For peptide/protein mw measurement |
| Infusion ESI-MS/MS |
Please call |
For protein identification and characterization |
| LC-ESI-MS |
Please call |
For mw measurement in complex sample |
| LC-ESI-MS/MS |
Please call |
For high throughput protein identification |
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| *Tube-gel digestion, Reference: Lu, et. al. Mol. & Cell. Proteomics, 2005, 4 (12): 1948-1958 |
